The Occurrence of a Nicotiana Glutinosa Haplont.

Acquired Systemic Susceptibility to Infection by Tobacco Mosaic Virus in Nicotiana glutinosa L

In Nicotiana glutinosa L. formation of local lesions on lower leaves inoculated with tobacco mosaic virus increased the susceptibility of the upper leaves to infection in a subsequent inoculation. The increase in susceptibility was detected as an increase of up to 3"5-fold in the number of lesions produced on the upper leaf and a corresponding increase in the amount of virus RNA synthesized. Th...

Diterpenes from Nicotiana glutinosa and their effect on fungal growth.

Determining the GmRIN4 Requirements of the Soybean Disease Resistance Proteins Rpg1b and Rpg1r Using a Nicotiana glutinosa-Based Agroinfiltration System

Rpg1b and Rpg1r are soybean disease resistance (R) genes responsible for conferring resistance to Pseudomonas syringae strains expressing the effectors AvrB and AvrRpm1, respectively. The study of these cloned genes would be greatly facilitated by the availability of a suitable transient expression system. The commonly used Niciotiana benthamiana-based system is not suitable for studying Rpg1b ...

Identification of essential active-site residues in ornithine decarboxylase of Nicotiana glutinosa decarboxylating both L-ornithine and L-lysine.

The cDNA encoding ornithine decarboxylase (ODC; EC 4.1.1.17), a key enzyme in putrescine and polyamine biosynthesis, has been cloned from Nicotiana glutinosa (GenBank AF 323910), and was expressed in Escherichia coli. The amino acid sequence of N. glutinosa ODC showed 90% identity with Datura stramonium ODC, and 44% identity with human ODC. N. glutinosa ODC did not possess the PEST sequence [a ...

Incorporation of carbon dioxide and acetate into the pyrrolidine ring of nicotine.

Glutamate, a proposed precursor of the pyrrolidine ring of nicotine, was isolated and degraded after metabolism of 14C02 for 6 hours by Nicotiana rustica and Nicotiana glutinosa and after metabolism of acetate-2J4C for 2 hours by N. rustica. The 14C distribution in glutamate from N. glutinosa after 6 hours of W02 metabolism was 20.2, 14.3, 13.9, 24.4, and 26.6%, in C-l through C-5, respectively...